| Cell Line Name |
GFP-NS0 stable cells |
| Catalog Number |
C3037 |
| Host Cell |
NS0, mouse myeloma cell line |
| Lot Number |
#0303 |
| Gene Sequence |
TurboGFP (GenBank: ASW25889.1) |
| Quantity |
Two vials of frozen cells (1x106 per vial) |
| Culture Medium |
RPMI with 10% FBS, 250 μg/ml Hygromycin B |
| Freezing Medium |
90% FBS and 10% DMSO |
| Storage |
Liquid nitrogen upon receipt |
| Product Datasheet: |
Download PDF |
THAWING AND CULTURING
• Remove the cell vial from liquid nitrogen tank and thaw cells quickly in a 37°C water bath
• Transfer the cells to a 15 ml centrifuge tube and slowly add 5 ml of pre-warmed complete growth medium
• Centrifuge the cells at 200x g for 5 min
• Remove the supernatant
• Resuspend cell pellet with 7 ml of complete growth medium and transfer cells to a T25 flask
• Incubate cells in an incubator with 5% CO2 at 37°C
• Split the cells twice a week or as needed.
DATA
a) Analysis of GFP expression by fluorescence microscopy
REFERENCES
Bueno-Solera, et al., Appl Microbiol Biotechnol. 2022 Aug;106(13-16):5007-5021.
