Accurus Biosciences provides a wide range of antibody and protein engineering solutions to optimize your antibody leads or proteins.

Antibody Engineering

• Expression optimization: to increase expression levels of your lead antibodies in CHO and HEK293 cells
• "Hot spot" removal: to remove undesired amino acid residues in the antibody CDR regions or frameworks (FR). These residues include:
• Unpaired cysteines
• Exposed N-link glycosylation sites
• Exposed deamidation sites
• D-linked isomerization sites
• Cleavage and hydrolysis sites
• Effector function optimization: to optimize the Fc mediated effector functions, such as CDC, ADCC, and phagocytosis
• Bispecific antibody designs and expression optimization: to design and produce bi-specific targeting molecules such as scFv-scFv, antibody-scFv, and heterodimeric IgGs. We use a high throughput CHO expression platform to evaluate the expression and functions of the bispecific molecules.

Protein Engineering

• Protein half-life extension: to increase protein half-life in vivo
• N-terminal and C-terminal Fc fusions
• N-terminal and C-terminal human serum albumin (HSA) fusions
• N-terminal and C-terminal fusion with synthetic PEGs
• Protein mutagenesis: to optimize protein function and stability
• Antibody-small protein fusions
• Protein epitope tagging: to design and produce proteins with various detection and purification tags including Fc, poly His, FLAG, HA, and Myc tags

Antibody & Protein Engineering Services