Stable cell line generation in MDCK II cells


Catalog Number Description Size Price
E3004 Stable cell line generation in MDCK II cells One gene $7500 Order


Work description:
  • Generation of expression construct encoding the protein of interest (gene synthesis for cDNA <500 bp included)
  • Transfection of MDCK II cells and selection of six individual stable clones
  • Evaluation of protein expression by FACS, western, or other methods
  • Preparation of frozen vials of cells for the highest expressing stable clone
Required materials:
  • Amino acid sequence or cDNA encoding the protein of interest
  • Antibody specific to the protein of interest
Final deliverables:
  • Frozen cell vials per customer's instructions
  • Product specification sheet
Timeline:
  • 4-6 weeks