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Stable cell pool generation in MDCK II cells
Services
Therapeutic Antibody Discovery
Stable cell pool generation in MDCK II cells
Catalog Number
Description
Size
Price
E3004
Stable cell pool generation in MDCK II cells
One gene
$8500
Order
Work description:
Generation of expression construct encoding the protein of interest (gene synthesis for cDNA <500 bp included)
Transfection of MDCK II
cells and selection of 3 individual stable pools
Evaluation of protein expression by FACS, western, or other methods
Preparation of frozen vials of cells for the highest expressing stable clone
Additional cost for subcloning for stable clones (please inquire)
Required materials:
Amino acid sequence or cDNA encoding the protein of interest
Antibody specific to the protein of interest
Final deliverables:
Frozen cell vials per customer's instructions
Product specification sheet
Timeline:
8-10 weeks