Stable cell pool generation in suspension NS0 cells


Catalog Number Description Size Price
E3003 Stable cell pool generation in suspension NS0 cells One gene $7500 Order


Work description:
  • Generation of expression construct encoding the protein of interest (gene synthesis for cDNA <500 bp included)
  • Transfection of NS0 cells and selection of up to three individual stable pools
  • Evaluation of protein expression by FACS, western, or other methods
  • Preparation of frozen vials of cells for the highest expressing stable pool 
Required materials:
  • Amino acid sequence or cDNA encoding the protein of interest
  • Antibody specific to the protein of interest
Final deliverables:
  • Frozen cell vials per customer's instructions
  • Product specification sheet 
Timeline:
  • 4-6 weeks
Additional services:
(Please inquire)
  • Single cell cloning of the stable pool