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Stable cell pool generation in adherent CHO-K1 or HEK293 cells
Services
Stable Cell Line Generation
Stable cell pool generation in adherent CHO-K1 or HEK293 cells
Catalog Number
Description
Size
Price
E3002
Stable cell pool generation in adherent CHO-K1 or HEK293 cells
One gene
$7500
Order
Work description:
Generation of expression construct encoding the protein of interest (gene synthesis for cDNA <500 bp included)
Transfection of adherent CHO-K1 or HEK293 cells and selection of 3 individual stable pools
Evaluation of protein expression by FACS, western, or other methods
Preparation of frozen vials of cells for the highest expressing stable pool
Additional cost for subcloning for stable clones (please inquire)
Required materials:
Amino acid sequence or cDNA encoding the protein of interest
Antibody specific to the protein of interest
Final deliverables:
Frozen cell vials per customer's instructions
Product specification sheetÂ
Timeline:
8-10 weeks
