Stable cell line generation in adherent CHO-K1 or HEK293 cells


Catalog Number Description Size Price
E3002 Stable cell line generation in adherent CHO-K1 or HEK293 cells One gene $7000 Order


Work description:
  • Generation of expression construct encoding the protein of interest (gene synthesis for cDNA <500 bp included)
  • Transfection of adherent CHO-K1 or HEK293 cells and selection of six individual stable clones
  • Evaluation of protein expression by FACS, western, or other methods
  • Preparation of frozen vials of cells for the highest expressing stable clone
Required materials:
  • Amino acid sequence or cDNA encoding the protein of interest
  • Antibody specific to the protein of interest
Final deliverables:
  • Frozen cell vials per customer's instructions
  • Product specification sheet 
Timeline:
  • 4-6 weeks