Recombinant protein stable production in CHO
(From DNA sequence to protein)


Catalog Number Description Size Price
E2203-0.1 Recombinant protein stable production in CHO (From DNA sequence to protein) 0.1 L $5265 Order
E2203-0.3 Recombinant protein stable production in CHO (From DNA sequence to protein) 0.3 L $5670 Order
E2203-0.6 Recombinant protein stable production in CHO (From DNA sequence to protein) 0.6 L $5950 Order
E2203-1 Recombinant protein stable production in CHO (From DNA sequence to protein) 1 L $6375 Order
E2203-2 Recombinant protein stable production in CHO (From DNA sequence to protein) 2 L $7760 Order
E2203-5 Recombinant protein stable production in CHO (From DNA sequence to protein) 5 L $9600 Order
E2203-B Recombinant protein stable production in CHO (From DNA sequence to protein) >5 L Please inquire Order


Work description:
  • Generation of expression constructs encoding the protein of interest fused with either human Fc or His tag 
    (other tags available for additional fee; gene synthesis for cDNA <500 bp)
  • Transfection of CHO cells and selection of up to three individual stable pools
  • Scale-up of the best pool for antibody production at requested scale
  • Protein purification from conditioned media using Protein A or Ni-NTA affinity chromatography
Required materials:
  • Amino acid sequences or cDNAs encoding the protein of interest
Final deliverables:
  • Purified protein in a buffer and concentration specified by the customer
  • Protein specification sheet or purification report
Timeline:
  • 4-6 weeks
Additional services:

(Please inquire)

  • Additional purification steps post Protein A affinity chromatography (e.g. IEX and SEC)
  • Size-exclusion chromatography (SEC) HPLC analysis to assess antibody purity and aggregation
  • Endotoxin level determination by LAL assay