Recombinant protein transient production in HEK293
(From DNA sequence to protein)


Catalog Number Description Size Price
E2103-0.1 Recombinant protein transient production in HEK293 (From DNA sequence to protein) 0.1 L $4200 Order
E2103-0.3 Recombinant protein transient production in HEK293 (From DNA sequence to protein) 0.3 L $4800 Order
E2103-0.6 Recombinant protein transient production in HEK293 (From DNA sequence to protein) 0.6 L $5500 Order
E2103-1 Recombinant protein transient production in HEK293 (From DNA sequence to protein) 1 L $6200 Order
E2103-B Recombinant protein transient production in HEK293 (From DNA sequence to protein) >1 L Inquire


Work description:
  • Generation of expression constructs encoding the protein of interest fused with either human Fc or His tag
    (other tags available for additional fee; gene synthesis for cDNA <500 bp included)
  • Protein expression in HEK293 cells by transient transfection at requested scale
  • Protein purification from conditioned media using Protein A or Ni-NTA affinity chromatography
Required materials:
  • Amino acid sequences or cDNAs encoding the protein of interest
Final deliverables:
  • Purified protein in a buffer and concentration specified by the customer
  • Protein specification sheet or purification report
Timeline:
  • 3-5 weeks
Additional services:

(Please inquire)

  • Additional purification steps post Protein A affinity chromatography (e.g. IEX and SEC)
  • Size-exclusion chromatography (SEC) HPLC analysis to assess antibody purity and aggregation
  • Endotoxin level determination by LAL assay