Work description: | - Generation of expression constructs encoding antibody heavy and light chains (VH and VL gene synthesis included)
- Transfection of CHO cells and selection of up to three individual stable pools
- Scale-up of the best pool for antibody production at requested scale
- Antibody purification using Protein A affinity chromatography
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Required materials: | - Amino acid sequences of VH and VL or cDNAs encoding VH and VL
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Final deliverables: | - Purified antibody in a buffer and concentration specified by the customer
- Protein specification sheet or purification report
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Timeline: | |
Additional services: (Please inquire)
| - Additional purification steps post Protein A affinity chromatography (e.g. IEX and SEC)
- Size-exclusion chromatography (SEC) HPLC analysis to assess antibody purity and aggregation
- Endotoxin level determination by LAL assay
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