ASSAN

Recombinant antibody stable production in CHO
(From DNA sequence to antibody)


Catalog Number Description Size Price
E2201-0.1 Recombinant antibody stable production in CHO (From DNA sequence to antibody) 0.1 L $6000 Order
E2201-0.3 Recombinant antibody stable production in CHO (From DNA sequence to antibody) 0.3 L $6500 Order
E2201-0.6 Recombinant antibody stable production in CHO (From DNA sequence to antibody) 0.6 L $7000 Order
E2201-1 Recombinant antibody stable production in CHO (From DNA sequence to antibody) 1 L $7500 Order
E2201-B Recombinant antibody stable production in CHO (From DNA sequence to antibody) >1 L Inquire
Work description:
  • Generation of expression constructs encoding antibody heavy and light chains (VH and VL gene synthesis included) 
  • Transfection of CHO cells and selection of up to three individual stable pools
  • Scale-up of the best pool for antibody production at requested scale
  • Antibody purification using Protein A affinity chromatography
Required materials:
  • Amino acid sequences of VH and VL or cDNAs encoding VH and VL
Final deliverables:
  • Purified antibody in a buffer and concentration specified by the customer
  • Protein specification sheet or purification report
Timeline:
  • 4-6 weeks
Additional services:

(Please inquire)

  • Additional purification steps post Protein A affinity chromatography (e.g. IEX and SEC)
  • Size-exclusion chromatography (SEC) HPLC analysis to assess antibody purity and aggregation
  • Endotoxin level determination by LAL assay