Recombinant antibody transient production in HEK293
(From DNA sequence to antibody)


Catalog Number Description Size Price
E2101-0.1 Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) 0.1 L $4200 Order
E2101-0.3 Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) 0.3 L $4800 Order
E2101-0.6 Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) 0.6 L $5500 Order
E2101-1 Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) 1 L $6200 Order
E2101-B Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) >1 L Inquire

Work description:

  • Generation of expression constructs encoding antibody heavy and light chains (VH and VL gene synthesis included)
  • Antibody expression in HEK293 cells by transient transfection at requested scale
  • Antibody purification using Protein A affinity chromatography

Required materials:

  • Amino acid sequences of VH and VL or cDNAs¬†encoding VH and VL

Final deliverables:

  • Purified antibody in a buffer and concentration specified by the customer
  • Protein specification sheet or purification report

Timeline:

  • 3-5 weeks

Additional services:

(Please inquire)

  • Additional purification steps post Protein A affinity chromatography (e.g. IEX and SEC)
  • Size-exclusion chromatography (SEC) HPLC analysis to assess antibody purity and aggregation levels
  • Endotoxin level determination by LAL assay