Recombinant antibody transient production in HEK293
(From DNA sequence to antibody)


Catalog Number Description Size Price
E2101-0.1 Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) 0.1 L $3465 Order
E2101-0.3 Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) 0.3 L $4320 Order
E2101-0.6 Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) 0.6 L $4590 Order
E2101-1 Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) 1 L $5100 Order
E2101-2 Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) 2 L $6880 Order
E2101-5 Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) 5 L $9600 Order
E2101-B Recombinant antibody transient production in HEK293 (From DNA sequence to antibody) >5 L Please inquire Order

Work description:

  • Generation of expression constructs encoding antibody heavy and light chains (VH and VL gene synthesis included)
  • Antibody expression in HEK293 cells by transient transfection at requested scale
  • Antibody purification using Protein A affinity chromatography

Required materials:

  • Amino acid sequences of VH and VL or cDNAs encoding VH and VL

Final deliverables:

  • Purified antibody in a buffer and concentration specified by the customer
  • Protein specification sheet or purification report

Timeline:

  • 3-5 weeks

Additional services:

(Please inquire)

  • Additional purification steps post Protein A affinity chromatography (e.g. IEX and SEC)
  • Size-exclusion chromatography (SEC) HPLC analysis to assess antibody purity and aggregation levels
  • Endotoxin level determination by LAL assay