Human LAG-3 CHO Stable Cells


Catalog Number Product Size Price
C1010 Human LAG-3 CHO Stable Cells 2 vials $6000 Order

Background Information


Lymphocyte-activation protein 3 (LAG-3) belongs to the immunoglobulin superfamily and shares sequence homology, exon/intron organization and chromosomal localization to CD4. LAG-3 cDNA encodes a 498 amino acid transmembrane protein with 4 extracellular Ig-like domains. The expression of LAG-3 is undetectable in resting peripheral blood lymphocytes but it is induced in activated T and NK cells. Human MHC Class II molecules can bind to LAG-3 and are considered as receptors for LAG-3 on B cells and dendritic cells. LAG-3 is proven to be an inhibitory receptor on activated T cells. Crosslinking of LAG-3 on activated T cells with an anti-LAG-3 antibody inhibits T cell proliferation and cytokine secretion. Anti-LAG-3 antibodies which blocked LAG-3 binding to MHC class II molecules had strong anti-tumor activity when used in combination with anti-PD-1 antibodies in tumor xerograph models.


Product Specifications



Catalog Number C1010
Cell Line Name CHO suspension cell pool expressing full-length human LAG-3 receptor
Gene Synonyms LAG-3, human
Gene Sequence Codon optimized from NP_002277.4 (Met1-Leu525)
Host Cell Suspension CHO
Quantity Two vials of frozen cells (10E7 cells/vial)
Stability >10 passages
Freeze Media Culture media with 10% DMSO
Storage Liquid nitrogen immediately upon receipt
Culture Medium 50% CD-CHO (Gibco#10743-029), 50% Ex-Cell CHO 5 Media (Sigma#C0363), supplemented with 8mM L-Glutamine, 1xHT, 1x Penn-Strep and 20ug/ml puromycin.
Mycoplasma Test Negative
Applications Antibody binding assays, IHC/Western blot analysis, or use as cell immunogen
Product Datasheet: Download PDF

Representative Data



Detection of human LAG-3 expression on human LAG-3 CHO Stable cells using a mouse monoclonal antibody specific for human LAG-3.


References


1.      Triebel F.et al., (2010) LAG-3, a novel lymphocyte activation gene closely related to CD4.. J Exp Med, 1990 May 1; 171(5):1393-405.

2.      Baixeras E.et al., (2011) Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens.. J Exp Med, 1992 Aug 1; 176(2):327-37.