Human CLDN18.1-FLAG-CHO-S Stable Cell Pool


Catalog Number Product Size Price
C1017 Human CLDN18.1-FLAG-CHO-S Stable Cell Pool 2 vials $6000 Order

Background Information


Claudin-18 (CLDN18) is a member of a large family of four-span transmembrane proteins called Claudins. These proteins are the essential components of the mammalian tight junctions (TJs) in epithelial cells. Claudin-18 has two splice variants, CLDN18.1 and CLDN18.2. While CLDN18.1 is specifically expressed in the lung tissue, CLDN18.2 expression in normal tissue is more restricted and is only detected in small patches of stomach mucosal. CLDN18.2 expression is elevated in many types of epithelial cancers including stomach, esophagus, pancreatic and ovarian cancers. The expression of CLDN18.2 is not only detected in primary tumors, but also in the metastatic sites. Therefore, CLDN18.2 is an ideal target for monoclonal antibody-based cancer therapies.


Product Specifications



Catalog Number C1017
Cell Line Name Chinese hamster ovary (CHO) suspension cell pool expressing full-length human CLDN18.1 (sorted)
Gene Synonyms CLDN18.1
Gene Sequence Codon optimized from Accession# NP_057453 with a FLAG tag (ADYKDDDK) inserted between V144 and T145.
Protein Structure Four span-transmembrane receptor
Host Cell Suspension CHO
Quantity Two vials of frozen cells (20x10EE6 per vial)
Stability >10 passages
Culture Media 50% CD-CHO (Gibco# 10743-029), 50% Ex-Cell CHO 5 Media (Sigma#C0363), supplemented with 8 mM L-Glutamine, 1x HT (Gibco# 11067030), 1x PenicillinStreptomycin, and 10µg/ml Puromycin.
Freezing Medium Culture medium with 10% DMSO
Storage Liquid nitrogen immediately upon arrival
Mycoplasma Test Negative
Applications Antibody binding assays, IHC/Western blot analysis, or use as cell immunogen
Product Datasheet: Download PDF

Representative Data



Detection of human CLDN18.1-FLAG expression on Human CLDN18.1-FLAG-CHO-S cells using a mouse monoclonal antibody specific for the FLAG tag (DYKDDDK), followed by staining with the PE-anti-mouse IgG.


Detection of human CLDN18.2 expression on Human CLDN18.1-FLAG-CHO-S cells using a human monoclonal antibody specific for human CLDN18.2, followed by staining with the PE-anti-human IgG. No CLDN18.2 is detected on the cells.


References


1.      Tureci O. et al. (2011) Claudin-18 Gene Structure, Regulation, and Expression is Evolutionary Conserved in Mammals. Gene, 481(2):83.

2.      Sahin, U. et al. (2008) Claudin-18 Splice Variant 2 is a Pan-Cancer Target Suitable for Therapeutic Antibody Development. Clin. Cancer Res. 14(23):7624.

3.   Niimi, T. et al. (2001) Claudin-18, A Novel Downstream Target Gene for the T/ENB/NKX2.1 Homerodomain Transcription Factor, Encodes Lung- and Stomach-Specific Isoforms Through Alternative Splicing. Mol. Cell. Biol. 21(21):7380.