Human CLDN18.2/MIA PaCa-2 Stable Cell Line


Catalog Number Product Size Price
C3002 Human CLDN18.2/MIA PaCa-2 Stable Cell Line 2 vials $8000 Order

Product Description:


Name:                                                    MIA PaCa-2 stable cell clone expressing full-length human  CLDN18.2 receptor

Gene Synonyms:                                 CLDN18.2

Gene Sequence:                                 Codon optimized from NP_001001026 (Met1-Val261)

Protein Structure:                               Four span-transmembrane receptor

Host Cell:                                             MIA PaCa-2, a human pancreatic carcinoma cell line

Quantity:                                              Two vials of frozen cells (106 cells/vial)

Stability:                                               >10 passages

Freeze Media:                                      90% FBS, 10% DMSO

Storage:                                                Liquid nitrogen immediately upon receipt

Mycoplasma Test:                              Negative

Applications:                                       Antibody binding assays, IHC/Western blot analysis, or use as cell immunogen

Product Datasheet/Protocols:       Download PDF



Background Information:


Claudin-18 (CLDN18) is a member of a large family of four-span transmembrane proteins called Claudins. These proteins are the essential components of the mammalian tight junctions (TJs) in epithelial cells. Claudin-18 has two splice variants, 18.1 and 18.2. While CLDN18.1 is specifically expressed in the lung tissue, CLDN18.2 expression in normal tissue is more restricted and is only detected in small patches of stomach mucosal. CLDN18.2 expression is elevated in many types of epithelial cancers including stomach, esophagus, pancreatic and ovarian cancers. The expression of CLDN18.2 is not only detected in primary tumors, but also in the metastatic sites. Therefore, CLDN18.2 is an ideal target for monoclonal antibody based cancer therapies.


Representative Data:


Detection of human CLDN18.2 expression on human CLDN18.2/MIA PaCa-2 stable cells using a monoclonal antibody specific for human CLDN18.2 at three different concentrations (2.5, 0.25, and 0.025ug/mL).


References:


1. Deer EL. et al. (2010): “Phenotype and genotype of pancreatic cancer cell lines”. Pancreas, 39(4), p425-35

2. Türeci O. et al. (2011): “Claudin-18 gene structure, regulation, and expression is evolutionary conserved in mammals”. Gene, 481(2), p83-92.

3. Sahin U. et al. (2008): “Claudin-18 Splice Variant 2 Is a Pan-Cancer Target Suitable for Therapeutic Antibody Development”. Clin. Cancer Res. 14 (23) p7624-7634.

4. Niimi T. et al. (2001): “Claudin-18, a Novel Downstream Target Gene for the T/EBP/NKX2.1 Homeodomain Transcription Factor, Encodes Lung- and Stomach-Specific Isoforms through Alternative Splicing”. Mol. Cell. Biol. 21(21), p7380-7390.