|C1001||Human CLDN18.2-CHO-S Stable Cell Pool||2 vials||$6000||Order|
Claudin-18 (CLDN18) is a member of a large family of four-span transmembrane proteins called Claudins. These proteins are the essential components of the mammalian tight junctions (TJs) in epithelial cells. Claudin18 has two splice variants, 18.1 and 18.2. While CLDN18.1 is specifically expressed in the lung tissue, CLDN18.2 expression in normal tissue is more restricted and is only detected in small patches of stomach mucosal. CLDN18.2 expression is elevated in many types of epithelial cancers including stomach, esophagus, pancreatic and ovarian cancers. The expression of CLDN18.2 is not only detected in primary tumors, but also in the metastatic sites. Therefore, CLDN18.2 is an ideal target for monoclonal antibody-based cancer therapies.
|Cell Line Name||Human CLDN18.2-CHO stable cells; CHO suspension cell pool expressing full-length human CLDN18.2 receptor|
|Gene Sequence||Codon optimized from NP_001001026 (Met1-Val261)|
|Protein Structure||Four span-transmembrane receptor|
|Host Cell||Suspension CHO|
|Quantity||Two vials of frozen cells (20x106 per vial)|
|Freeze Media||Culture medium with 10% DMSO|
|Culture Media||50% CD-CHO (Gibco # 10743-029), 50% Ex-Cell CHO 5 Media (Sigma#C0363), supplemented with 8 mM L-Glutamine, 1x HT (Gibco #11067030), 1x PenicillinStreptomycin, and 10 µg/ml Puromycin.|
|Applications||Antibody binding assays, IHC/Western blot analysis, or use as cell immunogen|
|Product Datasheet:||Download PDF|
Detection of human CLDN18.2 expression on human CLDN18.2-CHO Stable cells using a monoclonal antibody specific for human CLDN18.2
Tureci O. et al. (2011) “Claudin-18 Gene Structure, Regulation, and Expression is Evolutionary Conserved in Mammals”. Gene, 481(2):83.
Sahin, U. et al. (2008) “Claudin-18 Splice Variant 2 is a Pan-Cancer Target Suitable for Therapeutic Antibody Development”. Clin. Cancer Res. 14(23):7624.
Niimi, T. et al. (2001) “Claudin-18, A Novel Downstream Target Gene for the T/ENB/NKX2.1 Homerodomain Transcription Factor, Encodes Lung- and Stomach-Specific Isoforms Through Alternative Splicing”. Mol. Cell. Biol. 21(21):7380.