Human CLDN18.2-CHO Stable Cell Pool


Catalog Number Product Size Price
C1001 Human CLDN18.2-CHO Stable Cell Pool 2 vials $6000 Order

Product Description:


Name:                                                   CHO suspension cell pool expressing full-length human CLDN18.2 receptor

Gene Synonyms:                                CLDN18.2

Gene Sequence:                                 Codon optimized from NP_001001026 (Met1 - Val261)

Protein Structure:                               Four span-transmembrane receptor

Host Cell:                                             Suspension CHO

Quantity:                                              Two vials of frozen cells (20x10E6 per vial)

Stability:                                               >10 passages

Freeze Media:                                      Culture media with 10% DMSO

Storage:                                                Liquid nitrogen immediately upon arrival

Mycoplasma Test:                              Negative

Applications:                                       Antibody binding assays, IHC/Western blot analysis, or use as cell immunogen

Product Datasheet/Protocols:      Download PDF


Background Information:


Claudin 18 (CLDN18) is a member of a large family of four-span transmembrane proteins called Claudins. These proteins are the essential components of the mammalian tight junctions (TJs) in epithelial cells. Claudin-18 has two splice variants, 18.1 and 18.2. While CLDN18.1 is specifically expressed in the lung tissue, CLDN18.2 expression in normal tissue is more restricted and is only detected in small patches of stomach mucosal. CLDN18.2 expression is elevated in many types of epithelial cancers including stomach, esophagus, pancreatic and ovarian cancers. The expression of CLDN18.2 is not only detected in primary tumors, but also in the metastatic sites. Therefore, CLDN18.2 is an ideal target for monoclonal antibody-based cancer therapies.



Representative Data:


Detection of human CLDN18.2 expression on human CLDN18.2-CHO Stable cells using a mouse monoclonal antibody specific for human CLDN18.2.


       


References:



1.      Türeci O. et al. (2011) "Claudin-18 Gene Structure, Regulation, and Expression is Evolutionary Conserved in Mammals". Gene, 481(2):83.

2.      Sahin, U. et al. (2008) Claudin-18 Splice Variant 2 is a Pan-Cancer Target Suitable for Therapeutic Antibody Development". Clin. Cancer Res. 14(23):7624.

3.      Niimi, T. et al. (2001) "Claudin-18, A Novel Downstream Target Gene for the T/ENB/NKX2.1 Homeodomain Transcription Factor, Encodess Lung- and Stomach-Specific Isoforms Through Alternative Splicing". Mol. Cell. Biol. 21(21):7380.