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Recombinant Human CD47 Fc Chimera Protein

Recombinant Human CD47 Fc Chimera Protein


Catalog Number Product Size Price
P1012-200 Recombinant Human CD47 Fc Chimera Protein 200 µg $199 Order
P1012-500 Recombinant Human CD47 Fc Chimera Protein 500 µg $299 Order
P1012-B Recombinant Human CD47 Fc Chimera Protein Bulk Inquire

Product Specifications

Source: Human CD47 (Accession#AIC61946) extracellular domain (Gln19 – Pro 139) fused with human IgG1 Fc produced from HEK293 cells.
Structure: Disulfide-linked homodimer
Predicted N-terminal: Gln 19
Predicted Molecular Weight: 39.6 kDa, reducing conditions
Apparent Molecular Weight on SDS-PAGE: 55 kDa, reducing conditions
Formulation: 0.22 µm filtered protein solution in 1x PBS 
Storage: < -20°C
Estimated Purity: >95% as determined by SDS-PAGE
Protein Endotoxin Level: Not measured
Protein Aggregation:

<5% as determined by SDS-PAGE


Product Description

Application:Bioassay (biochemical assay)
Product Description:CD47 is a 40-60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Human CD47 is an integral membrane protein that consists of a 123 amino acid (aa) extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and a 34 aa C-terminal cytoplasmic tail (3). Alternative splicing of human CD47 generates additional isoforms with deletions in the cytoplasmic tail (3). Within the N-terminal ECD, human CD47 shares 63% amino acid sequence identity with mouse and rat CD47. A portion of the N?terminal ECD can by shed from smooth muscle cells by MMP-2-mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5). These interactions prevent macrophage-mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5-8). The CD47-SIRP alpha interaction is species specific, and this lack of cross-species interaction has been implicated in xenotransplantation rejection. CD47 associates in cis with Fas on T cells and enhances Fas?mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses. CD47 also associates in cis with Integrins  alpha 4 beta 1, alpha V beta 3, alpha 2b beta 3, and  alpha 2 beta 1 which can positively or negatively modulate Integrin?mediated function (2).  On dendritic cells and myeloma cells, CD47 ligation by TSP-1 induces giant cell formation and osteoclast differentiation.
Other Names:Integrin-Associated Protein (IAP), OA3
References:
  1. Sarfati, M. et al. (2009) Curr. Drug Targ. 9:842.
  2. Isenberg, J.S. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:615.
  3. Campbell, I.G. et al. (1992) Cancer Res. 52:5416
  4. Maile, L.A. et al. (2008) Mol. Endocrinol. 22:1226
  5. Oldenborg, P. -A. et al. (2000) Science 288:2051.
  6. Lui, Y. et al. (2002) J. Biol. Chem. 277:10028.
  7. Stefanidakis, M. et al. (2008) Blood 112:1280.
  8. deVries, H.E. et al. (2002) J. Immunol. 168:5832.
Product Datasheet:Download PDF

Data


SDS-PAGE Analysis

10 µg/lane of huCD47 Fc Chimera was resolved on SDS-PAGE gel in non-reducing (NR) and reducing (R) conditions and visualized by coomassie blue staining.