Recombinant Human CD33 Fc Chimera Protein


Catalog Number Product Size Price
P1003-100 Recombinant Human CD33 Fc Chimera Protein 100 µg $229 Order
P1003-200 Recombinant Human CD33 Fc Chimera Protein 200 µg $439 Order
P1003-B Recombinant Human CD33 Fc Chimera Protein Bulk Inquire

Product Specifications


Source: Human CD33 (Accession# NP_AAH28152.1) extracellular domain (Met 17 – Val 258) fused with human IgG1 produced from HEK293 cells.
Structure: Disulfide-linked homodimer
Predicted N-terminal: Met 17
Predicted Molecular Weight: 52.8 kDa, reducing conditions
Apparent Molecular Weight on SDS-PAGE: 70 kDa, reducing conditions
Formulation: 0.22 µm filtered protein solution in PBS
Storage: < -20°C
Estimated Purity: >95% as determined by SDS-PAGE
Protein Endotoxin Level: Not measured
Protein Aggregation: <10% as determined by SDS-PAGE

Product Description


Application: Bioassay (biochemical assay)
Product Description: CD33 (cluster of differentiation 33) is a member of the immunoglobulin superfamily. It is a myeloid-specific member of the sialic acid-binding immunoglobulin-related lectin family which also includes CD22, sialoadhesin, myelin-associated glycoprotein, and siglecs 5, 7, and 8. Human CD33 is a 67 kDa transmembrane cell surface glycoprotein receptor with only one V-set and one C2-set Ig-like domains (1). The extracellular domain of CD33 binds alpha-2,3-linked sialic acid residues in N- and O-glycans on the cell surface (2). It can mediate sialic acid-dependent cell interactions as a recombinant protein (3). Studies have shown that in the presence of pervanadate, a phosphatase inhibitor, CD33 is tyrosine-phosphorylated and recruits SHP-1 and SHP-2 phosphatases, suggesting that CD33 functions as an inhibitory receptor (4).
Other Names: Siglec-3, FLJ00391, P67
References:
  1. Freeman S. D. et al. (1995) Blood. 85:2005.
  2. Kelm S and Schauer R. (1997) Int Rev Cytol. 175137.
  3. Tanimoto M. et al. (1989) Leukemia 3:339.
  4. Sujatha P.P. et al. (2000) Blood. 96:483.
Product Datasheet: Download PDF

Data


SDS-PAGE Analysis

10 µg/lane of human CD33 Fc Chimera was resolved on SDS-PAGE gel in non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.