Catalog Number | Product | Size | Price | |
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P1003-100 | Recombinant Human CD33 Fc Chimera Protein | 100 µg | $229 | Order |
P1003-200 | Recombinant Human CD33 Fc Chimera Protein | 200 µg | $439 | Order |
P1003-B | Recombinant Human CD33 Fc Chimera Protein | Bulk | Inquire |
Source: | Human CD33 (Accession# NP_AAH28152.1) extracellular domain (Met 17 – Val 258) fused with human IgG1 produced from HEK293 cells. |
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Structure: | Disulfide-linked homodimer |
Predicted N-terminal: | Met 17 |
Predicted Molecular Weight: | 52.8 kDa, reducing conditions |
Apparent Molecular Weight on SDS-PAGE: | 70 kDa, reducing conditions |
Formulation: | 0.22 µm filtered protein solution in PBS |
Storage: | < -20°C |
Estimated Purity: | >95% as determined by SDS-PAGE |
Protein Endotoxin Level: | Not measured |
Protein Aggregation: | <10% as determined by SDS-PAGE |
Application: | Bioassay (biochemical assay) |
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Product Description: | CD33 (cluster of differentiation 33) is a member of the immunoglobulin superfamily. It is a myeloid-specific member of the sialic acid-binding immunoglobulin-related lectin family which also includes CD22, sialoadhesin, myelin-associated glycoprotein, and siglecs 5, 7, and 8. Human CD33 is a 67 kDa transmembrane cell surface glycoprotein receptor with only one V-set and one C2-set Ig-like domains (1). The extracellular domain of CD33 binds alpha-2,3-linked sialic acid residues in N- and O-glycans on the cell surface (2). It can mediate sialic acid-dependent cell interactions as a recombinant protein (3). Studies have shown that in the presence of pervanadate, a phosphatase inhibitor, CD33 is tyrosine-phosphorylated and recruits SHP-1 and SHP-2 phosphatases, suggesting that CD33 functions as an inhibitory receptor (4). |
Other Names: | Siglec-3, FLJ00391, P67 |
References: |
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Product Datasheet: | Download PDF |
10 µg/lane of human CD33 Fc Chimera was resolved on SDS-PAGE gel in non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.