Recombinant Human CTLA-4 Ig (Fc) Chimera


Catalog Number Product Size Price
P1001-100 Recombinant Human CTLA-4 Ig (Fc) Chimera 100 µg $149 Order
P1001-500 Recombinant Human CTLA-4 Ig (Fc) Chimera 500 µg $389 Order
P1001-B Recombinant Human CTLA-4 Ig (Fc) Chimera Bulk Inquire

Product Specifications


Source: Human CTLA-4 (Accession# NP_005205.2) extracellular domain (Ala37-Phe162) fused with human IgG1 Fc produced from HEK293 cells.
Structure: Disulfide-linked homodimer
Predicted N-terminal: Ala 37
Predicted Molecular Weight: 39.5 kDa (monomer)
Apparent Molecular Weight on SDS-PAGE: 47.0 kDa, reducing conditions
Formulation: 0.22 µm filtered protein solution in PBS
Storage: < -20°C
Estimated Purity: >95% as determined by SDS-PAGE
Protein Endotoxin Level: Not measured
Protein Aggregation: No obvious protein aggregates

Product Description


Application: Bioassay
Product Description: CTLA-4 (cytotoxic T lymphocyte-associated molecule-4) is a T cell surface glycoprotein and a member of the immunoglobulin superfamily. The 223-amino acid human protein contains a leader sequence, a single extracellular V domain, a transmembrane domain, and a cytoplasmic tail encoded by 4 exons, respectively (1). It is a disulfide-linked homodimer in the extracellular domain at cysteine residue 120 (2). CD28 is structurally related to CTLA-4, with CTLA-4 sharing 27% (murine) to 31% (human) amino acid identity with CD28 (1). Both CTLA-4 and CD28 bind to B7-1 (CD80) and B7-2 (CD86) on antigen presenting cells, with CTLA-4 binding at a 20- to 50-fold greater affinity than CD28 does (3). CD28 delivers an activation signal to T cells while CTLA-4 transmits an inhibitory signal to T cells (4). CD28 is found in considerable amounts on the cell surface of the majority of resting T cells whereas CTLA-4 presents significantly lower expression, predominantly appearing after T cell activation (5).
Other Names: CD152, ALPS5, CELIAC3, GRD4, GSE, IDDM12
References:
  1. Harper, K. et al. (1991) J. Immunol. 147:1037.
  2. Linsley, P.S. et al. (1995) J. Biol. Chem. 270:15417.
  3. van der Merwe, P.A. et al. (1997) J. Exp. Med. 185:393.
  4. Krummel, M.F. and Allison, J.P. (1995) J. Exp. Med. 182:459.
  5. Alegre, M.L. et al. (1996) J. Immunol. 157:4762.
Product Datasheet: Download PDF

Data


SDS-PAGE Analysis

10 µg/lane of human CTLA-4 Ig was resolved on SDS-PAGE gel in non-reducing (NR) and reducing (R) conditions and visualized by coomassie blue staining.