Human LILRB1-CHO-K1 Stable Cell Line

Catalog Number Product Size Price
C3027 Human LILRB1-CHO-K1 Stable Cell Line 2 vials $2000 Order


Cell Line Name Human LILRB1-CHO-K1 Stable Cell Line
Catalog Number C3027
Gene Sequence Q8NHL6 (Full-length cDNA)
Host Cell Adherent CHO-K1
Quantity Two vials of frozen cells (2x106 per vial)
Culture Medium DMEM with 10% FBS, 4 μg/ml puromycin
Freezing Medium 90% FBS and 10% DMSO
Storage Liquid nitrogen upon receipt
Product Datasheet: Download PDF


Leukocyte immunoglobulin-like receptor B1 (LILRB1), also known as leukocyte immunoglobulin-like receptor 8 (LIR-8), is a type I transmembrane protein belonging to the immunoglobulin superfamily. It is expressed on the surface of various immune cells, including monocytes, macrophages, dendritic cells, and natural killer (NK) cells. LILRB1 is characterized by its extracellular domain, which contains four immunoglobulin-like domains and two cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). Through its ITIMs, LILRB1 recruits tyrosine phosphases to inhibit the activity of immune cells like NK cells, monocytes, macrophages, and T and B cells. LILRB1 has been shown to play a role in various biological processes, including phagocytosis, cytokine production, and immune tolerance. In cancer, LILRB1 expression is increased leading to tumor progression, immune evasion and metastasis. Specifically, LILRB1 binds to tumor cells expressing beta 2 microglobulin complexed with MHC class I, allowing an escape from T cell recognition. Given its natural inhibitory function, LILRB1 is immunosuppressive and indirectly tumor supporting. Therefore studies suggest that targeting LILRB1 or disrupting the MHC class I-LILRB1 signaling axis can stop tumor progression in cancer, making LILRB1 a focus as a therapeutic target for cancer treatment.


• Remove the cell vial from liquid nitrogen tank and thaw cells quickly in a 37°C water bath

• Transfer the cells to a 15 ml centrifuge tube and slowly add 5 ml of pre-warmed complete growth medium

• Centrifuge the cells at 200x g for 5 min

• Remove the supernatant

• Resuspend cell pellet with 7 ml of complete growth medium and transfer cells to a T25 flask

• Incubate cells in an incubator with 5% CO2 at 37°C

• Split the cells twice a week or as needed.


Detection of human LILRB1 expression on CHO-K1 cells using PE-anti human LILRB1 antibody (BioLegend #333708)


Barkal AA, Weiskopf K, Kao KS, Gordon SR, Rosental B, Yiu YY, George BM, Markovic M, Ring NG, Tsai JM, McKenna KM, Ho PY, Cheng RZ, Chen JY, Barkal LJ, Ring AM, Weissman IL, Maute RL. Engagement of MHC class I by the inhibitory receptor LILRB1 suppresses macrophages and is a target of cancer immunotherapy. Nat Immunol. 2018 Jan;19(1):76-84. doi: 10.1038/s41590-017-0004-z. Epub 2017 Nov 27. PMID: 29180808; PMCID: PMC5832354.

Deng M, Chen H, Liu X, Huang R, He Y, Yoo B, Xie J, John S, Zhang N, An Z, Zhang CC. Leukocyte immunoglobulin-like receptor subfamily B: therapeutic targets in cancer. Antib Ther. 2021 Feb 9;4(1):16-33. doi: 10.1093/abt/tbab002. PMID: 33928233; PMCID: PMC7944505.