GFP-NS0 stable cells

Catalog Number Product Size Price
C3037 GFP-NS0 stable cells 2 vials $2000 Order


Cell Line Name GFP-NS0 stable cells
Catalog Number C3037
Host Cell NS0, mouse myeloma cell line
Lot Number #0303
Gene Sequence TurboGFP (GenBank: ASW25889.1)
Quantity Two vials of frozen cells (1x106 per vial)
Culture Medium RPMI with 10% FBS, 250 μg/ml Hygromycin B
Freezing Medium 90% FBS and 10% DMSO
Storage Liquid nitrogen upon receipt
Product Datasheet: Download PDF


• Remove the cell vial from liquid nitrogen tank and thaw cells quickly in a 37°C water bath
• Transfer the cells to a 15 ml centrifuge tube and slowly add 5 ml of pre-warmed complete growth medium
• Centrifuge the cells at 200x g for 5 min
• Remove the supernatant
• Resuspend cell pellet with 7 ml of complete growth medium and transfer cells to a T25 flask
• Incubate cells in an incubator with 5% CO2 at 37°C
• Split the cells twice a week or as needed.


a) Analysis of GFP expression by fluorescence microscopy


Bueno-Solera, et al., Appl Microbiol Biotechnol. 2022 Aug;106(13-16):5007-5021.