Human CLDN18.2-Luciferase-MIA PaCa2 stable cell line

Catalog Number Product Size Price
C3035 Human CLDN18.2-Luciferase-MIA PaCa2 stable cell line 2 vials $6000 Order

Background Information

Claudin-18 (CLDN18) is a member of a large family of four-span transmembrane proteins called Claudins. These proteins are the essential components of the mammalian tight junctions (TJs) in epithelial cells. Claudin-18 has two splice variants, 18.1 and 18.2. While CLDN18.1 is specifically expressed in the lung tissue, CLDN18.2 expression in normal tissue is more restricted and is only detected in small patches of stomach mucosal. CLDN18.2 expression is elevated in many types of epithelial cancers including stomach, esophagus, pancreatic and ovarian cancers. The expression of CLDN18.2 is not only detected in primary tumors, but also in the metastatic sites. Therefore, CLDN18.2 is an ideal target for monoclonal antibody-based cancer therapies.

Product Specifications

Catalog Number C3035
Cell Line Name Human CLDN18.2-Luciferase-MIA PaCa2 stable cell line
Gene Sequence Human CLDN18.2: NP_001001026 (Met1-Val261); Firefly luciferase: AFE85520.1
Host Cell MiaPaca2, human pancreatic carcinoma cell line
Quantity Two vials of frozen cells (~2x106 per vial)
Culture Medium DMEM with 10% FBS, 1ug/ml puromycin, and 250 ug/ml hygromycin
Freezing Medium 90% FBS and 10% DMSO
Storage Liquid nitrogen upon receipt
Product Datasheet: Download PDF

Representative Data

(A) Detection of human CLDN18.2 expression on human CLDN18.2-Luc-MiaPaca2 stable cells using a monoclonal antibody specific for human CLDN18.2 (Cat #A1008)

(B) Luciferase activity measurement for human CLDN18.2-Luc-MiaPaca2 stable cells


Türeci O. et al. (2011) “Claudin-18 gene structure, regulation, and expression is evolutionary conserved in mammals”. Gene, 481(2), p83-92.

Sahin U. et al. (2008) “Claudin-18 Splice Variant 2 Is a Pan-Cancer Target Suitable for Therapeutic Antibody Development”. Clin. Cancer Res. 14 (23) p7624-7634.

Niimi T. et al. (2001) “Claudin-18, a Novel Downstream Target Gene for the T/EBP/NKX2.1 Homeodomain Transcription Factor, Encodes Lung- and Stomach-Speci?c Isoforms through Alternative Splicing”. Mol. Cell. Biol. 21(21), p7380-7390.