|C3008||Human CLDN18.1-FLAG-CHO-K1 Stable Cell Line||2 vials||$2300||Order|
Claudin-18 (CLDN18) is a member of a large family of four-span transmembrane proteins called Claudins. These proteins are the essential components of the mammalian tight junctions (TJs) in epithelial cells. Claudin-18 has two splice variants, 18.1 and 18.2. While CLDN18.1 is specifically expressed in the lung tissue, CLDN18.2 expression in normal tissue is more restricted and is only detected in small patches of stomach mucosal.
|Cell Line Name||Human CLDN18.1-FLAG-CHO-K1 Stable Cell Line|
|Gene Sequence||Full-length human CLDN18.1 (NP_057453)|
|Host Cell||CHO-K1 (Adherent cells)|
|Quantity||Two vials of frozen cells (~1x106 per vial)|
|Culture Medium||DMEM with 10% FBS and 4 µg/ml puromycin|
|Freezing Medium||90% FBS and 10% DMSO|
|Storage||Liquid nitrogen upon receipt|
|Product Datasheet:||Download PDF|
A. Detection of human CLDN18.1 expression on CHO-K1/Human CLDN18.1 cells using a mouse monoclonal antibody specific for the Flag tag. (Cat. #A1001)
B. Detection of human CLDN18.2 expression on CHO-K1/Human CLDN18.1 cells using a human monoclonal antibody specific for human CLDN18.2. (Cat #A1008)
No CLDN18.2 is detected on the cells.
Türeci O. et al. (2011) “Claudin-18 gene structure, regulation, and expression is evolutionary conserved in mammals”. Gene, 481(2), p83-92.
Sahin U. et al. (2008) “Claudin-18 Splice Variant 2 Is a Pan-Cancer Target Suitable for Therapeutic Antibody Development”. Clin. Cancer Res. 14 (23) p7624-7634.
Niimi T. et al. (2001) “Claudin-18, a Novel Downstream Target Gene for the T/EBP/NKX2.1 Homeodomain Transcription Factor, Encodes Lung- and Stomach-Speci?c Isoforms through Alternative Splicing”. Mol. Cell. Biol. 21(21), p7380-7390.