Human CLDN18.2-KATO III stable cell pool

Catalog Number Product Size Price
C3016 Human CLDN18.2-KATO III stable cell pool 2 vials $2000 Order

Background Information

Claudin-18 (CLDN18) is a member of a large family of four-span transmembrane proteins called Claudins. These proteins are the essential components of the mammalian tight junctions (TJs) in epithelial cells. Claudin-18 has two splice variants, 18.1 and 18.2. While CLDN18.1 is specifically expressed in the lung tissue, CLDN18.2 expression in normal tissue is more restricted and is only detected in small patches of stomach mucosal. CLDN18.2 expression is elevated in many types of epithelial cancers including stomach, esophagus, pancreatic and ovarian cancers. The expression of CLDN18.2 is not only detected in primary tumors, but also in the metastatic sites. Therefore, CLDN18.2 is an ideal target for monoclonal antibody-based cancer therapies.

KATO III is a human gastric carcinoma cell line which expresses lower levels of human CLDN18.2. We stably transfected full length human CLDN18.2 into KATO III cells and generated stable cell pools which express more than 100 fold higher expression of human CLDN18.2 comparing to the parental cells

Product Specifications

Catalog Number C3016
Cell Line Name Human CLDN18.2-KATO III stable cell pool
Gene Sequence Full-length human CLDN18.2 (NP_001001026)
Host Cell KATO III (Semi-adherent)
Quantity Two vials of frozen cells (~1x106 per vial)
Culture Medium DMEM with 10% FBS and 0.125 µg/ml puromycin
Freezing Medium 90% FBS and 10% DMSO
Storage Liquid nitrogen upon receipt
Product Datasheet: Download PDF

Representative Data

Detection of human CLDN18.2 expression on human CLDN18.2-KATO III cells (Cat. #C3016) using recombinant anti-huCLDN18.2 mAbs D10C (Cat. #A1008) and E9 (Cat. #A1013), followed by staining with a secondary PE-anti-human IgG antibody


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Niimi T. et al. (2001) “Claudin-18, a Novel Downstream Target Gene for the T/EBP/NKX2.1 Homeodomain Transcription Factor, Encodes Lung- and Stomach-Speci?c Isoforms through Alternative Splicing”. Mol. Cell. Biol. 21(21), p7380-7390.