Human CD47-CHO-K1 stable cell line


Catalog Number Product Size Price
C3017 Human CD47-CHO-K1 stable cell line 2 vials $6000 Order

Background Information


CD47 is a transmembrane protein that belongs to the immunoglobulin (Ig) superfamily and regulates phagocytosis by macrophages. Binding of CD47 to its counter-receptor, SIRP?? on macrophages leads to inhibition of phagocytosis. CD47 is widely expressed in normal tissues but is highly expressed on cell surface of many types of cancer, including human acute myeloid leukemia (AML) and small-cell lung cancer (SCLC). CD47 serves as a don’t-eat-me signal and its overexpression is a mechanism for cancer cells to evade immune surveillance. Blocking the interaction of CD47 with SIRP? by anti-CD47 blocking antibody leads to increased phagocytosis and tumor growth inhibition.


Product Specifications



Catalog Number C3017
Cell Line Name Human CD47-CHO-K1 stable cell line
Gene Sequence Full-length human CD47 (NP_001768.1)
Host Cell CHO-K1 (Adherent cells)
Quantity Two vials of frozen cells (~1x106 per vial)
Culture Medium DMEM with 10% FBS and 4 µg/ml puromycin
Freezing Medium 90% FBS and 10% DMSO
Storage Liquid nitrogen upon receipt
Product Datasheet: Download PDF

Representative Data



Detection of human CD47 expression on CHO-K1 cells using a recombinant human anti-CD47 monoclonal antibody (Cat. #A1011) specific for human CD47, followed by staining with a PE-anti-human IgG antibody.


References


1.      McCracken MN, Cha AC, Weissman IL. Molecular pathways: activating T cells after cancer cell phagocytosis from blockade of CD47 "don't eat me" signals. Clin Cancer Res. 21:3597-601. 2015.

2.      Liu, J. et al., Pre-clinical development of a humanized anti-CD47 antibody with anti-cancer therapeutic potential. PLOS ONE 10(9):e0137345, 2015.