Human CD40 ligand (CD40L)-EL4 stable cell pool


Catalog Number Product Size Price
C1004 Human CD40 ligand (CD40L)-EL4 stable cell pool 2 vials $6000 Order

Background Information


CD40 ligand, CD40L (also known as CD154, TRAP or gp39), is a 261 amino acid type II transmembrane glycoprotein belonging to the TNF family, CD40L is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, like NK cells, mast cells, basophils and eosinophils. Human CD40L shares 78% amino acid identity with its murine counterpart. The receptor of CD40L is CD40, a type I transmembrane glycoprotein belonging to the TNF receptor family. CD40 is expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelium. Although all monomeric, dimeric and trimeric forms of soluble CD40L can bind to CD40, the trimeric form of soluble CD40L has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40L mediates a range of activities on B cells including induction of activation-associated surface antigen, entry into cell cycle, isotype switching and Ig secretion and memory generation. CD40-CD40L interaction also plays important roles in monocyte activation and dendritic cell maturation.


Product Specifications



Catalog Number C1004
Cell Line Name Human CD40 ligand (CD40L)-EL4 stable cell pool
Gene Synonyms Human CD40 ligand or CD40L, CD154, TNFSF5, TRAP, gp39
Gene Sequence P29965.1
Host Cell Mouse EL-4
Quantity Two vials of frozen cells (5E6 cells/vial)
Stability >10 passages
Freeze Media Culture media with 10% DMSO
Storage Liquid nitrogen immediately upon receipt
Culture Medium RPMI with 10% FBS and 1 ?g/ml puromycin
Freezing Medium 90% FBS and 10% DMSO
Storage Liquid nitrogen
Mycoplasma Test Negative
Applications Antibody binding analysis by flow cytometry, IHC, or western blot; CD40:CD40L interaction analysis; In vitro B cell stimulation
Product Datasheet: Download PDF

Representative Data



A. Detection of human CD40L expression on human CD40L-EL-4 stable cells using a mouse monoclonal antibody specific for human CD40L (BioLegendCat. #310805).

B. Detection of human CD40 binding to human CD40L-EL-4 stable cells using a human CD40 ECD-Fc protein followed by staining with PE-anti-human IgG.


References


1.      Hollenbaugh, D., Grosmaire, L. S., Kullas, C. D., Chalupny, N. J., Braesch-Andersen, S., Noelle, R. J., Stamenkovic, I., Ledbetter, J. A., Aruffo, A.The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity.EMBO J. 11: 4313-4321, 1992.

2.      Graf, D., Korthauer, U., Mages, H. W., Senger, G., Kroczek, R. A.Cloning of TRAP, a ligand for CD40 on human T cells.Europ. J. Immun. 22: 3191-3194, 1992.

2.      Gauchat, J.-F., Aubry, J.-P., Mazzei, G., Life, P., Jomotte, T., Elson, G., Bonnefoy, J.-Y.Human CD40-ligand: molecular cloning, cellular distribution and regulation of expression by factors controlling IgE production.FEBS Lett. 315: 259-266, 1993.

2.      Straw, A. D., MacDonald, A. S., Denkers, E. Y., Pearce, E. J.CD154 plays a central role in regulating dendritic cell activation during infections that induce Th1 or Th2 responses.J. Immun. 170:727-734, 2003.