Recombinant Human IgG4 Isotype Control

Catalog Number Product Size Price
A1004-100 Recombinant Human IgG4 Isotype Control 100 µg $210 Order
A1004-1000 Recombinant Human IgG4 Isotype Control 1 mg $450 Order

Product Specifications

Catalog Number A1004
Isotype Human IgG4, κ
Molecular Weight 150kDa
Expression Host Human HEK293
Purification Purified by affinity chromatography
Purity >95%
Formulation 0.22µm filtered in PBS, pH6.8; no preservative
Concentration 4.2mg/mL
Endotoxin level < 0.002EU/µg of protein as determined by LAL assay
Storage <-20°C
Application Isotype control for flow cytometry, ELISA, functional assays, in vivo animal studies
Product Description There are four types of human immunoglobulin G (IgG): IgG1, IgG2, IgG3, and IgG4. The human IgG4 isotype control, G03C51, is a recombinant human IgG4 antibody. It can be used as an isotype control for human IgG4 antibodies in various in vitro and in vivo assays.
Product Datasheet: Download PDF

Representative Data

A: Human IgG4 isotype control (5µg/lane) was resolved on SDS-PAGE gel in non-reducing (NR) and reducing (R) conditions and visualized by Coomassie blue staining.

B: Size Exclusion Chromatography (SEC) Analysis of human IgG4 isotype control.

C: Anti-human CD47-huIgG4 mAb and human IgG4 isotype control (Cat. #A1004) were tested for binding to CD47-expressing cells by flow cytometry. Cells were stained with PE-anti-human IgG secondary antibody.