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Mouse PD-1-CHO-K1 stable cell line


Catalog Number Product Size Price
C3095 Mouse PD-1-CHO-K1 stable cell line 2 vials $3950 Order

Product Specifications


Catalog Number C3095
Cell Line Name Mouse PD-1-CHO-K1 stable cell line
Accession Number NM_008798.3
Host Cell Adherent CHO-K1
Quantity Two vials of frozen cells (2x106 per vial)
Culture Medium DMEM with 10% FBS, 4 µg/ml puromycin
Freezing Medium 90% FBS and 10% DMSO
Storage Liquid nitrogen upon receipt
Product Datasheet: Download PDF

Representative Data


Detection of mouse PD-1 expression on mouse PD-1-CHO-K1 stable cells (A) and Vector Control-CHO-K1 (B) using a monoclonal antibody specific for mouse PD-1 (eBioscience #12-9985-81)


Background


PD-1 (Programmed Cell Death Protein 1) is a cell surface receptor protein belonging to the immunoglobulin superfamily. PD-1 is primarily expressed on the surface of activated T cells, B cells, natural killer cells (NK cells), and monocytes. The primary function of PD-1 is to inhibit T-cell activation and effector functions. When PD-1 interacts with its ligands, PD-L1 (Programmed Death-Ligand 1) and PD-L2 (Programmed Death-Ligand 2), which are expressed on the surface of antigen-presenting cells (APCs) and certain non-immune cells, it sends inhibitory signals to T cells. This inhibitory signaling pathway helps prevent autoimmune responses and limits collateral damage to healthy tissues during immune responses. Many cancer cells upregulate PD-1 and its ligands inhibiting T cell activity thus, creating an immunosuppressive microenvironment allowing cancer cells to thrive and escape immune destruction. PD-1 and its associated pathway have emerged as a promising therapeutic target in the field of cancer immunotherapy. PD-1 inhibitors have shown great success in treating cancer by blocking PD-1’s interaction with its ligands, T cells can recognize and attack cancer cells correctly.

References

Chen DS, Irving BA, Hodi FS. Molecular pathways: next-generation immunotherapy—inhibiting programmed death-ligand 1 and programmed death-1. Clin Cancer Res. 18(24):6580-6587. 2012.

Hargadon KM, Johnson CE, Williams CJ. Immune checkpoint blockade therapy for cancer: An overview of FDA-approved immune checkpoint inhibitors. Int Immunopharmacol. 62:29-39. 2018